Recombinant Human CDC25A, Transfected HEK293 Cell Lysate 230-10312-100 from RayBiotech

Product Specs
ItemRecombinant Human CDC25A, Transfected HEK293 Cell Lysate
CompanyRayBiotech
Price Pricing Info Supplier Page View Company Product Page
Catalog Number230-10312-100
Quantity100 ug
ConcentrationDetermined by BCA protein assay
Conjugate/TagN-terminal his-tag
FormatLyophilized powder
PurityUnpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20,000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control
Molecular WeightRecombinant protein product has a calculated molecular mass of 61. The actual molecular weight may increase slightly due to the potential post-translational modifications (PTMs)
SpeciesHuman
Source/Expression SystemHuman embryonic kidney 293 (HEK293) cells
NCBI Full Gene Namecell division cycle 25A
NCBI Gene AliasesCDC25A2